Oxidative DNA damage measured in human lymphocytes: large differences between sexes and between countries, and correlations with heart disease mortality rates

• DOI: 10.1096/fasebj.12.13.1397
• Publication Date: 2018-01-18T12:01:00Z
• Authors: Andrew R. Collins; Catherine M. Gedik; Begoña Olmedilla; Susan Southon; Mary Bellizzi
• Keywords: Adult; Male; 0301 basic medicine; Heart Diseases; Lutein; Deoxyguanosine; Ascorbic Acid; Middle Aged; Carotenoids; Chemoprevention; Antioxidants; 3. Good health; Europe; 03 medical and health sciences; Lycopene; 8-Hydroxy-2'-Deoxyguanosine; Neoplasms; Ethnicity; Humans; Female; Lymphocytes; Oxidation-Reduction; DNA Damage

The 'antioxidant hypothesis' proposes that vitamin C, E, carotenoids, and other antioxidants occurring in fruit vegetables afford protection against heart disease cancer by preventing oxidative damage to lipids DNA, respectively. To test elements of this hypothesis, we have measured blood levels dietary antioxidants, 8-oxodeoxyguanosine (8-oxo-dG) concentrations lymphocyte healthy men women from five European countries: France, Ireland, Netherlands, Spain, the U.K. Volunteers, aged 25–45, all nonsmokers, gave samples before after a 12-wk carotenoid supplementation regime. Vitamin C was plasma E carotenoids were serum high-performance liquid chromatography (HPLC). 8-oxo-dG assayed HPLC (with coulometric detection) DNA isolated lymphocytes same samples. Mean values calculated for groups volunteers at each sampling time according country, sex, (between 9 24 individual contributing mean). We found vary significantly sex country. A low mean concentration is seen countries, France Spain. higher (up about threefold) Ireland Oxidative not affected supplementation; nor there any association with baseline which are generally similar countries. countries sampled lie on an axis northern southern Europe steep gradient terms premature disease. There strong between coronary mortality (r = 0.95, P < 0.01). Women rates, do correlate 8-oxo-dG. In deaths, only colorectal shows significant positive correlation 0.91, 0.05), stomach negatively correlated oxidation −0.92, 0.01).—Collins, A. R., Gedik, C. M., Olmedilla, B., Southon, S., Bellizzi, M. human lymphocytes: large differences sexes correlations rates. FASEB J. 12, 1397–1400 (1998)