Thedaf‐2gene network for longevity regulates oxidative stress resistance and Mn‐superoxide dismutase gene expression inCaenorhabditis elegans

• DOI: 10.1096/fasebj.13.11.1385
• Publication Date: 2018-01-25T09:36:43Z
• Authors: Yoko Honda; Shuji Honda
• Keywords: Oxidative Stress; 0303 health sciences; 03 medical and health sciences; Gene Expression Regulation; Superoxide Dismutase; Longevity; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Receptor, Insulin; Signal Transduction

Longevity is regulated by the daf-2 gene network in Caenorhabditis elegans. Mutations gene, which encodes a member of insulin receptor family, confer life extension (Age) phenotype and constitutive dauer (a growth-arrested larval form specialized for dispersal) formation phenotype. The Age mutually potentiated two mutations clk-1 homologue yeast CAT5/COQ7 known to regulate ubiquinone biosynthesis. In this study, we demonstrated that mutation also conferred an oxidative stress resistance (Oxr) phenotype, was enhanced mutation. Similar Oxr genetic pathway insulin-like signaling from daf-16 HNF-3/forkhead transcription factor. These findings led us examine whether regulates expression antioxidant defense enzymes. We found mRNA level sod-3 Mn-superoxide dismutase (SOD), much higher mutants than wild type. Moreover, increased pathway. Although mutant itself did not display phenotypes, them mutant, suggesting involved longevity ways: composes original program program. observations suggest controls regulating Mn-SOD-associated system. This system appears play role efficient maintenance at stage.