Membrane Biogenesis in B‐lymphocytes

• DOI: 10.1096/fasebj.20.5.a947-b
• Publication Date: 2021-06-22T00:08:07Z
• Authors: Paolo Fagone; Rungtawan Sriburi; Joseph W. Brewer; Suzanne Jackowski
• Keywords: Phosphatidylethanolamine

B-lymphocytes expand the membrane network for synthesis and secretion of antibodies. We investigated mechanisms that drive biogenesis during differentiation CH12 B-cells. Phosphatidylcholine (PtdCho) phosphatidylethanolamine (PtdEtn) content per cell doubled after induced by LPS. PtdCho acquired shorter chain monounsaturated fatty acids, whereas PtdEtn remained a reservoir polyunsaturated species. Alteration composition was accounted increased transcription Fasn Acac in first phase gene regulation. Elevated expression Elovl6 occurred second phase, Scd2 upregulated final third phase. Increased driven elevated kinase expression, Chka Etnk1, Expression rate-limiting step synthesis, Pcyt2, amplified Pcyt1 which encodes CCTα, unchanged. Metabolic labelling demonstrated CCTα activity corresponding to affinity lipids isolated at times following redistributed with majority associated extracellular membranes. These data indicate biophysical properties were major determinant stimulating activity. Enzymes acid degradation downregulated differentiation, Acox3, Crot, Ech1, Echs1. describe mechanism B-cell as initiated transcriptional upregulation de novo phospholipid synthesis. (Research support GM45737, ALSAC.)