Probiotic Kefir Antihypertensive Effects in Spontaneously Hypertensive Rats Involves Central and Peripheral Mechanisms

• DOI: 10.1096/fasebj.2018.32.1_supplement.924.2
• Publication Date: 2021-06-21T16:38:04Z
• Authors: Mirian A. Silva‐C...; Sarah C. Peaden; Francesca E. Mowry; Henri A.G. Ducray; Ludmila P. Globa; Iryna B. Sorokulova; Tadeu U. Andrade; Vinicia C. Biancardi
• Keywords: Dysbiosis; Kefir

Gut microbiota dysbiosis has been associated with hypertension. Long‐term use of probiotics have shown to decrease blood pressure, improve baroreflex, and ameliorate endothelial dysfunction in Spontaneously Hypertensive Rats (SHR). Yet, the mechanisms are not well understood. We aimed evaluate peripheral centrally mediated by which probiotic kefir promotes beneficial effects SHRs. used Wistar Kyoto (WKY) SHR male rats, divided into: WKY treated vehicle, milk fermented grains (5%; SHR‐Kefir; oral gavage, daily/9 weeks). evaluated pressure tail cuff method weekly. The were on gut anatomy (histological analysis small intestine), composition (microbiological feces), endotoxin levels (Lipopolysaccharide (LPS) content analyzed serum). Within paraventricular nucleus hypothalamus (PVN), we microglia activation tyrosine hydroxylase (TH) protein expression via immunofluorescence. TH mRNA real time PCR. SHR‐Kefir presented a lower mean (151.9±2.9mmHg) compared (173.1±2.41mmHg, P<0.0001 n=6). Basal SHRs differed from that observed for WKY, suggesting dysbiosis. Treatment restored normal profile Lactobacillus, Staphylococcus, Brucella Bifidobacterium spp . number Paneth cells/crypt was decreased (1.66±0.06) (2.40±0.08 p<0.0004), an effect normalized treatment (SHR‐Kefir:2.23±0.15). An increase LPS serum (0.71±0.02) (0.54±0.04 EU/mL p<0.01), increased intestinal permeability during hypertension, reversed (0.54±0.01). PVN, density as indication activation. found (9.47±0.61) (6.87±0.68 p<0.07), rats (4.77±0.55, arbitrary units [AU]). overexpression reduced (WKY: 5.25±0.58; SHR: 13.68±0.81 SHR‐Kefir: 8.42±0.55, AU p<0.0001). within PVN (punches) 47%. Kefir 1.21±0.45; 1.79±0.50; 1.30±0.27 fold change). Our data shows restores structure, diminishes endotoxin, reduces neuroinflammation, balances hypothalamus. Altogether, our suggests antihypertensive‐associated involves microbiota– brain axis communication Support or Funding Information This work funded CAPES PDSE 88881.133261/2016‐01 MAS. VCB is AHA 14SDG20400015. abstract Experimental Biology 2018 Meeting. There no full text article this published FASEB Journal