The Role of Fasting Interventions on Skeletal Muscle in High Fat Diet Fed Mice

• DOI: 10.1096/fasebj.2019.33.1_supplement.537.6
• Publication Date: 2021-06-22T00:43:21Z
• Authors: Melissa Puppa; Aaron Persinger; Katie Brown; Ryan Wible; Sunita Sharma; Nicholas Smith; Jade Caldwell; Marie van der Merwe; Richard Bloomer
• Keywords: Intermittent Fasting; Dietary fat

Obesity is a growing epidemic worldwide leading to rates of chronic disease and diabetes. A diet rich in omega‐6 fatty acids refined sugars, known as the “Western diet,” may be one contributing factor. Prevention weight gain, well loss, have been shown beneficial preventing obesity‐associated disease. Growing evidence suggests that fasting reliable method aid loss prevention gain. Although effective reducing fat mass, there risk losing lean muscle. There are many different types fasting, including time restricted feeding alternate day fasting. Little about effects these fasts on skeletal muscle while continuing consume high diet. The purpose this study was examine Mice were fed (45% fat, mostly lard) for 6 weeks subset mice consumed standard chow At end weeks, continued with ad libitum access food, remaining divided into following groups: (HFD), (SC), purified (PD), caloric restriction 80% consumption (CR), 6h (TRF), days (ADF). kept their respective diets 8 additional euthanized at hindlimb muscles weighed snap frozen analysis. HFD gained more mass than groups p<0.001. no significant difference normalized tibia length across all groups; however, relation body weight, had significantly less mass. ADF (p<0.001) TRF preserved weight. Muscle IGF‐1 mRNA expression decreased HFD. unable attenuate decrease; changes composition DF attenuated expression. These data support use various interventions obesity prevent still adipose Support or Funding Information University Memphis School Health Studies This abstract from Experimental Biology 2019 Meeting. full text article associated published FASEB Journal .