Heterocellular contact can dictate arterial function

• DOI: 10.1096/fasebj.2019.33.1_supplement.682.4
• Publication Date: 2021-06-22T00:51:54Z
• Authors: Claire A. Ruddiman; Xiaohong H. Shu; T.C. Stevenson Ke...; Alexander S. Keller; Yang Yang; Miranda E. Good; Angela K. Best; Linda Columbus; Brant E. Isakson
• Keywords: Electrical impedance myography; Hyperpolarization

Resistance arteries use different dilatory heterocellular signaling mechanisms than conduit (endothelial derived hyperpolarization versus nitric oxide). Anatomically, resistance myoendothelial junctions (MEJs), endothelial cell (EC) extensions that make contact with smooth muscle cells (SMCs), which are not present in arteries. The objective of this study was to determine if the presence MEJs can alter signaling. We previously demonstrated plasminogen activator inhibitor‐1 (PAI‐1) regulate formation MEJs. Thus, we applied pluronic gel containing PAI‐1 directly (carotid arteries, CAs) live mice could induce found a significant increase EC projections resemble MEJs, correlating increased biocytin dye transfer from ECs SMCs and dilation NS309. Next, used pressure myography investigate whether these structural changes were accompanied by functional change vasodilatory Interestingly, PAI‐1‐treated CAs underwent switch artery profile via endothelial‐derived (EDH) diminished oxide (NO) Following application, also carotid expression alpha globin, protein predominantly expressed Carotids lacking globin (Hba1−/−) L‐NAME, an inhibitor NO signaling, able prevent vasodilation. or absence is important determinant for influence communication. In particular, our data suggests may allow EDH predominate Support Funding Information This work supported HL088554 (BEI) National Natural Science Foundation China 81672945 (XHS). abstract Experimental Biology 2019 Meeting. There no full text article associated published FASEB Journal .