Ultrasound evaluation of cardiovascular function in male and female APOE3 and APOE4 mice

• DOI: 10.1096/fasebj.2021.35.s1.04684
• Publication Date: 2021-06-11T00:39:09Z
• Authors: Zi Liu; Ashley Christensen; Brikena Gusek; Johana Vallejo‐Elias; Jessica Powell; Thomas Virden; T Jones; Carleton Jones; Delrae Eckman
• Keywords: Apolipoprotein E

Introduction Alzheimer's disease (AD) is the most common form of dementia and 6th leading cause death in US. Growing evidence supports a link between AD cardiovascular (CVD). Variant isoforms apolipoprotein E (APOE) are risk factors for both CVD. The APOE isoform, APOE3, has neutral developing AD, whereas APOE4 increases risk. Pathogenic alterations cardiac (C), aortic (TA) carotid artery (CA) function known to contribute neurodegenerative diseases including AD. Previous studies our lab have demonstrated cerebral arteries from APOE3 mice exhibit “normal” aging characteristics, exhibited delay age-related changes vascular structure function. Because this observation, we investigated whether structure/function (in vivo) mirrored vitro experiments isolated arteries. Here, evaluated structure/function, expressing human-ApoE targeted replacement (B6.129P2-Apoetm2(APOE*3)Mae N8) or (B6.129P2-Apoetm3(APOE*4)Mae N8)(Taconic Labs). We hypothesized that would delayed compared age matched mice. Methods In vivo high-resolution ultrasound (U/S) imaging was performed evaluate cardiac, thoracic aorta (TA), (CA), blood flow young (3-4 months), adult (12-14 months) aged (18-20 Male female were used. Blood pressure (BP) determined using tail-cuff method. All U/S BP values collected under isoflurane anesthesia. Results considered significant at p<0.05. Both hAPOE3 hAPOE4 increase body weight. peak velocity measurements posterior showed no statistical difference groups. CA assessed by wall thickness (WT) pulse wave (PWV) respectively. Our data an PWV WT TA also (P<0.05). indices function, output (CO), ejection fraction (EF), stroke volume (SV) left ventricle mass increased with And finally, systolic diastolic significance Conclusion These suggest show similar changes. Future been designed assess psycho-social stress and/or diet involved allele related development CVD AD/dementia.