Characterizing the Correlation Between Angiogenesis and Osteogenesis In Vivo Using Multicontrast Functional Imaging in a Calvarial Defect Model

• DOI: 10.1096/fasebj.2022.36.s1.r2091
• Publication Date: 2022-05-03T09:18:13Z
• Authors: Yunke Ren; Akanksha Bhargava; Janaka Senarathna; Xinying Chu; Warren Grayson; Arvind Pathak
• Keywords: Ex vivo; X-ray microtomography; Bone remodeling

Angiogenesis is a key factor in bone healing that allows the delivery of oxygen, nutrients, inflammatory and precursor cells to defect site. It induces dramatic structural functional remodeling vasculature during first 4 weeks healing. Although recent advances optical imaging have elucidated vivo relationship between angiogenesis osteogenesis calvarial model, these efforts were mostly limited bone. Therefore, better characterize changes vascular function (i.e. blood flow, oxygenation, etc.) cascade we developed multicontrast framework assess microvascular architecture using intrinsic signal (IOS) imaging; flow with laser speckle contrast (LSC) formation bright-field at high spatial (5 µm) temporal (200 ms) resolutions. With this system acquired images from 10 animals defects every 2 days, over (Fig. 1a, d). The then digitally segmented IOS for quantitative analysis 1b, c). We also euthanized an animal each week assessing 3D volume ex vivo, high-resolution (10 CT workflow recently called VascuViz 2a, b, Using framework, demonstrated angiogenic within microenvironment was most robust post injury day (D) D10, which vessel length, density all showed significant increase 2c). exhibited sudden D6 when started, vessels perfused D12 1d). These peaked by end plateaued next correlated large smaller last 2e). results indicate both strongly correlate growth early stages Next, plan include additional indices such as maturity intravascular well image-based hemodynamic models further role osteogenesis. believe novel characterizing can be utilized inform design tissue engineering (TE) constructs craniofacial regeneration. Collectively, herald new era "image-based TE".