Does Dietary Methionine Alter Perivascular Adipose Tissue Function?

• DOI: 10.1096/fasebj.2022.36.s1.r4091
• Publication Date: 2022-05-03T09:09:39Z
• Authors: Davis Lee; Adam Corken; Elizabeth C. Wahl; Keshari M. Thakali
• Keywords: Calorie

Perivascular adipose tissue (PVAT) is an depot surrounding blood vessels and a paracrine regulator of vascular tone. Obesity associated with the loss PVAT-mediated anti-contractile properties, thus contributing to obesity-associated hypertension. Hydrogen sulfide (H2 S) gaseous signaling molecule that produced in vasculature important for regulating In PVAT, cystathionine gamma lyase (Cse) primary source H2 S which acts as adipose-derived relaxing factor. Dietary methionine restriction recapitulates many metabolic effects caloric leads preferential expression activating transcription factor 4 (ATF4) facilitates Cse transcription. We hypothesized dietary will prevent high fat diet-induced PVAT through activation integrated stress response (ISR) increased ATF4-mediated Cse. Male Wistar rats were fed either control (C, 10% calories from fat) or diet (HFD) (H, 60% was replete (R, 0.86% methionine) depleted (D, 0.12% 12 weeks such there four groups: CR, CD, HR, HD. prevented body weight gain, while gained over week feeding period. There no difference gain between HFD rats. Arterial pressure, measured conscious using tail cuff plethysmography, not different groups After on diets, arterial contractility assessed wire myography 2nd order mesenteric arteries (MAs) without attached PVAT. our hands, presence reduced contraction norepinephrine (NE, 10 µM) greater relaxation acetylcholine (ACh,10 MAs all groups, properties. ACh NE-precontracted MAs, had effect ACh-induced relaxation. conclusion, male rats, modulation MA contractility, profound small Future studies include examining gene protein other ATF4 responsive genes real time qPCR, Western blot analysis, immunohistochemical staining cross-sections. These provide further insights into interventions minimize cardiovascular co-morbidities obesity.