Potential 1H NMR urinary metabolomic biomarkers for Lung cancer in hamster model

• DOI: 10.1096/fasebj.25.1_supplement.984.1
• Publication Date: 2021-06-21T16:01:09Z
• Authors: ARVIND GOJA; Andreea Geamanu; Nadia Sadaat; Smiti Gupta
• Keywords: Sarcosine

The objective of this study was to use the metabolomic approach investigate early potential biomarkers for lung cancer. Golden Syrian hamsters were randomized into 2 groups. Lung cancer induced in treatment group by a chemical carcinogen, N‐nitrosobis‐2‐(oxopropyl) amine (BOP). Proton nuclear magnetic resonance ( 1 H NMR) spectra, acquired using 500 MHz magnet processed ACD labs software. processed, digitized NMR spectral data analyzed multivariate analysis software, SIMCA P+. Principal Component Analysis (PCA) score plots showed significant separation between and control metabolites responsible differences two groups as observed from PCA loading plots, quantified CHENOMX metabolite database. (n=26) found be significantly different traced back their respective biochemical pathways an effort gain mechanistic insight progression view point. Some these such glycine, sarcosine, alanine have been previously reported increased other types. These could potentially serve general. In addition, some may specific relevance therefore further explored possible aggressive type. Grant Funding Source : This work funded start up funds Wayne State University.