Blood pressure profile and response to NG ‐nitro‐L‐arginine methyl ester challenge in conscious TRPV4‐deficient mice

• DOI: 10.1096/fasebj.26.1_supplement.1056.9
• Publication Date: 2021-06-21T15:33:05Z
• Authors: Yoshinori Nishijima; Hayley Lund; Xiaodong Zheng; David Mattson; David Zhang
• Keywords: TRPV4; Mean arterial pressure; Concomitant; Basal (medicine)

Endothelial TRPV4 is a Ca 2+ entry channel critically involved in endothelium‐mediated dilation both conduit and resistance vascular beds. However the importance of this TRPV4‐mediated vasodilation vivo has not been well characterized. We investigated role blood pressure (BP) regulation under normal conditions response to hypertensive challenge. The study was performed TRPV4‐deficient (TRPV4 −/− ) wild‐type (WT) mice implanted with radiotelemetry device for continuous recording mean arterial (MAP), heart rate (HR) activity. Basal MAP tends be lower (101 ± 3 mmHg, n=3, p=0.054) than WT controls (108 1 n=4), whereas HR comparable (565 11 bpm) (571 8 bpm). Hypertension induced by administration L‐NAME (0.5 g/L), nitric oxide synthase inhibitor, drinking water 7 days. L‐NAME‐induced increase similar (9.8 1.6 %) (9.5 %), although concomitant reduction less (−7.5 1.1 (−12.6 1.9 %). activity (4.1 0.8 units) compared (6.3 1.5 units). In conclusion, exhibit trend toward basal BP but retain an These findings call further evaluation significance tone vivo.