Acoustic trauma reduces SLC4A11 expression in the mouse cochlea

• DOI: 10.1096/fasebj.26.1_supplement.694.7
• Publication Date: 2021-06-21T15:39:47Z
• Authors: Hiroshi Yamomoto; Teresa Wilson; Irina Omelchenko; Yuan Zhang; Tsutomu Nakashima; Xiaorui Shi; Alfred L. Nuttall
• Keywords: Homeostasis; Hypoxia

Rationale The solute carrier 4A11 (SLC4A11) protein, a plasma membrane bound sodium‐borate co‐transporter, is expressed in many different organs including salivary glands, kidney, cornea and the inner ear where it functions to maintain ion homeostasis. Its functional importance underscored by causative role of SLC4A11 gene mutations several inherited diseases, all which are characterized some form sensorineural defect. Objective To compare expression with other members SLC4 family ear, eye kidney examine affect acoustic trauma on its cochlea. Methods Results By quantitative real‐time PCR immunoblot analysis, transcript protein levels whole cochlea were markedly higher relative whereas SLC4A2 SLC4A7 was quantitatively comparable among three organs. Acoustic resulted significant decrease cochlear lateral wall. Further studies cultured endothelial cells chemical hypoxia mimetics revealed that down‐regulation can be mediated inducible factor. Conclusion Hypoxia, major source damage tissues from trauma, may mechanism responsible for reduced SLC4A11. Supported grants 5R01DC000105 (ALN), 1R01DC010844 (XS), P30DC005983.