Stimulation of adenosine A1 receptor inhibits the basolateral 50 pS K channels in the thick ascending limb of rat kidney

• DOI: 10.1096/fasebj.26.1_supplement.867.5
• Publication Date: 2021-06-21T15:25:31Z
• Authors: wennan li; Lijun Wang; Shumin Kong; Peng Wu; Mingxiao Wang; Haiyan Luan; Li Gu; Lili Fan; Ruimin Gu
• Keywords: Adenosine A1 receptor; Adenosine kinase; Adenosine receptor antagonist; Adenosine A3 receptor

We used the patch‐clamp technique to study effect of stimulation Adenosine A 1 receptor on basolateral 50 pS K channels in thick ascending limb (TAL) rat kidney. 100nM or 200nM (ADO) inhibited channels. However, 400nM, 800nM and 1μM did not decrease channel activity. Adenosine's was mimicked by specific adenosine agonist Cyclohexyladenosine (CHA). Inhibition with 8‐cyclopentyl‐1, 3‐dipropylxanthine (DPCPX), antagonist, abolished inhibitory adenosine. Stimulation activity presence inhibitor phospholipase C (PLC), U‐73122 protein kinase‐C (PKC), Calphostin‐C, respectively. The mitogen‐activated kinase ( MAPK) inhibitors PD98059 (ERK) SB202190 (p38) increased CHA. CHA decreased SB202190. These studies show that channels, coupled receptor, is mediated PLC‐PKC MAPK pathways work supported Chinese National Natural Science Foundation 30770800, 31071017 31171110.