Visualizing the Effect of Iron on the Localization of Intracellular Ferritin in Mosquito Cells

• DOI: 10.1096/fasebj.27.1_supplement.1009.5
• Publication Date: 2021-06-16T10:07:01Z
• Authors: Zachary Ryan Conley; Dawn Geiser; Joy Winzerling
• Keywords: Organelle; Ferroportin; Blood meal

Ferritin is a 24‐subunit molecule, made up of heavy chain (HCH) and light (LCH) homologues that stores controls the release dietary iron in mammalian, plant, insect systems. Dietary taken blood meal stored inside ferritin, previous work mosquitoes demonstrates an important function for process egg development. One approach to limit spread arthropod‐borne diseases, such as yellow fever, West Nile virus malaria, reduce number potential vectors by interfering with this mosquito reproduction thereby decrease transmission rates. The localization ferritin subunits CCL‐125 ( Aedes aegypti ) 4a3b Anopheles gambiae cells under various treatment conditions further elucidate regulation metabolism these disease observe dynamics intracellular following administration. Deconvolution microscopy was used capture 3D fluorescent images treated visualize LCH subunit multiple focal planes follow‐up detection HCH subunit. Fluorescent probes were illuminate cell organelles (i.e. Golgi apparatus, lysosomes, endoplasmic reticulum, nuclei), while secondary demonstrated abundance co‐localization within organelles. These will help develop model biochemical perhaps advance novel hypotheses crucial role reproduction. Agricultural Experiment Station College Agriculture Life Sciences supported research.