Effects of leptin in the retrotrapezoid nucleus (RTN) on CO2‐sensitivity and respiration.

• DOI: 10.1096/fasebj.27.1_supplement.1137.12
• Publication Date: 2021-06-21T14:59:09Z
• Authors: Mirian Bassi; Werner Issao Furuya; José Vanderlei Me...; Débora Simões de ...; Jussara Márcia do...; Alexandre da Silva; John Edward Hall; Ian C Wenker; Daniel Mulkey; Eduardo Colombari
• Keywords: Leptin receptor; Energy homeostasis

Evidence suggests that, in addition to regulate energy homeostasis, leptin also contributes the CNS control of breathing response changes CO 2 /H + (i.e., central chemoreception). However, cellular targets leptin‐dependent drive breathe have not been elucidated. Here, we tested effects on hypercapnic ventilatory deficient mice (ob/ob: 42±2 g) vivo , and ‐responsiveness RTN neurons astrocytes brain slices isolated from rat pups (P7–12). We found that 3‐ day unilateral injections (1 μg/100 nl, n=7/group) increased responsiveness 7% ob/ob (4378±294 μl.min −1 .g vs. 2849±319 vehicle group). Immunohistochemical analysis using antibodies for receptors, (aldehyde dehydrogenase 1L1) (biocytin) suggest receptors are expressed by but mice. acute vitro exposure (10 min, 50 nM) elicited no effect excitability chemosensitive (cell‐attached current clamp) or conductance ‐sensitive (whole‐cell voltage clamp; Ihold −80 mV). These results although long‐term application into respiratory mice, molecular mechanisms remain unclear.