Acute Administration of n‐3 Triglyceride Emulsion Provides Marked Cardioprotection After Ischemia/Reperfusion

• DOI: 10.1096/fasebj.27.1_supplement.359.6
• Publication Date: 2021-06-21T15:08:33Z
• Authors: Hylde Zirpoli; Mariane Abdillahi; Nosirudeen Quadri; Radha Ananthakris...; Zhengbin Zhu; LingJie Wang; Qing Li; Richard J Deckelbaum; Ravichandran Rama...
• Keywords: Cardioprotection; Ex vivo; Creatine kinase

n‐3 fatty acids may decrease cardiovascular disease risk. We questioned if acute intervention of triglyceride (TG) emulsion (48% = EPA+DHA) is protective and improves cardiac function after ischemia/reperfusion (I/R). TG were administered I/R in two models: a) hearts (C57BL/6 mice) perfused ex‐vivo using the Langendorff technique (LT); b) with occlusion left anterior descending coronary artery (LAD) vivo . After LAD (1.5g/kg body weight) was injected intraperitoneally at end ischemia 1h later. In LT model I/R, perfusion KREBS‐Hensleit buffer (KH) led to markedly decreased ventricular developed pressure (LVDP) (by 60%) arrhythmias, but reperfusion KH+ (300mg/100ml) improved LVDP recovery. model, near normal echo‐and electrocardiograms maintained 48hrs infarct size reduced by 80% treated animals vs control. For both models, markers injury, lactate dehydrogenase creatine kinase, significantly mice. increased a marker apoptosis Bcl‐2 50%), autophagy beclin 1 HIF1 80%). conclude that an injection provides cardioprotection. This provide novel therapy myocardial infarction humans. Grant Funding Source : Nutrition (ASN)