Expression of the mouse Lin28a gene is epigenetically regulated by histone modification

• DOI: 10.1096/fasebj.27.1_supplement.550.5
• Publication Date: 2021-06-16T10:27:52Z
• Authors: Alan L.Y. Pang; Alexandra C. Title; Owen M. Rennert
• Keywords: Trichostatin A

The RNA‐binding protein Lin28a is considered to be a “stemness” marker. expressed predominantly in pluripotent cells and cancer cells. However, the regulation of its expression has not been thoroughly studied. We found that promoter DNA was transcriptionally active non ‐expressing cells, which made us hypothesize regulated epigenetically. By bisulfite‐sequencing analysis, we did observe correlation between absence gene methylation. Consistently, activated non‐ expressing by 5‐aza‐2′‐deoxycytidine treatment. Instead, treatment NIH/3T3 with histone deacetylase inhibitor trichostatin A (TSA) induced sensitive nuclease digestion, suggesting mediated acetylation relaxation chromatin. significant enrichment H3K9Ac reduced occupancy H3K9Me2 detected on opposite pattern modifications observed when silenced. modification also influenced abundance RNA polymerase II promoter. Taken together, our results indicate mouse epigenetically modification.