Atmospheric pressure plasma induced cell cycle arrest in human aortic endothelial cells

• DOI: 10.1096/fasebj.27.1_supplement.916.8
• Publication Date: 2021-06-16T10:21:54Z
• Authors: Eunyoung Shim; Bomi Gweon; Hyeonyu Kim; Sanghoo Park; Wonho Choe; Jennifer Hyunjong...
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

There has been a great deal of interests in application of atmospheric pressure plasma (APP) for biomedical and clinical uses such as cancer therapy and wound healing. However, most of these studies have been limited to adhesion and apoptosis of cells induced by APP in epithelial cells. In this study, we investigated the effects of a moderate APP (970 V, 50 kHz, 20 min) on the human aortic endothelial cells (HAECs). Some phenotypic changes were observed in the APP treated HAECs. Dramatic reduction of the cell population was observed which indicates the cellular cyclic arrest. To determine the involved mechanism of the changes in proliferation rate, immunofluorescence assay was used to analyze cyclin B1, p21, and p53 using the real‐time PCR. In addition to the decrease in the 2‐D migration speed, tube assay for the APP treated HAECs showed a decrease in the mean tube length when compared to that of the untreated cells. Our results support the potential use of APP to control the proliferation of the endothelial cells for anti‐angiogenic therapies for cancers.