cDNA and lincRNA microarrays: identification of expression patterns related to metastatization in oral squamous cell carcinoma (1048.6)

• DOI: 10.1096/fasebj.28.1_supplement.1048.6
• Publication Date: 2021-06-15T17:01:30Z
• Authors: Joao Paulo Olivei...; Mabel Gigliola; Mariana Macedo; Dirce Carraro; Fernando Soares
• Keywords: Primary tumor

Oral squamous cell carcinoma (OSCC) metastatic cascade still a poorly understood mechanism, in terms of gene expression differences between primary site and cells at lymph nodes. Using 20 matched samples OSCC cases, being 10 from sites metastastic nodes we performed lincRNA cDNA microarrays analysis (Agilent`s Sureprint G3 Human Genome V2.0). Our results showed 617 genes lincRNAs significantly differentially expressed tumors. Integrated pathway alteration canonical pathways such as Ras homolog family, member A signaling (RhoA pathway; p=0.002) tumor necrosis factor receptor 2 (TNFR2 p=0.009). The most up‐regulated included SNORA65 (9,045‐fold), ZMAT1 (4,776‐fold) HNRNPU‐AS1 (4,626‐fold), RNU12 (4,557‐fold), TLE4 (4,555‐fold), the down‐regulated MYBPH (‐67,818‐fold), ACTA1 (‐58,551‐fold), TNNC1 (‐23,119‐fold), F2RL2 (‐17,431‐fold) MYLPF (‐13,785‐fold). Regarding lincRNAs, MALAT1 was overexpressed nodes, with 4.2‐fold increase. study shows existence several different patterns also points to novel genes, which has not yet been implicated metastasis, providing new potential targets oral cancer research. Grant Funding Source : Supported by FAPESP grants 2011/18606‐7‐0 2010/08400‐0