Suppression of cardio‐protective molecules by diabetic marker miR‐29: a mechanism for the high rate of cardiac damage in diabetes? (1086.6)

• DOI: 10.1096/fasebj.28.1_supplement.1086.6
• Publication Date: 2021-06-15T17:01:30Z
• Authors: Nicholas Arnold; Purushotham Koppula; Lakshmi Pulakat
• Keywords: Diabetic Cardiomyopathy; Masson's trichrome stain

Diabetic marker miRNA‐29 (miR‐29) is up‐regulated by pro‐inflammatory cytokines and hyperglycemia. miR‐29 induced suppression of MCL‐1, a protein essential for mitochondrial function cell survival, occurs in pancreatic β‐cell death. We hypothesized that conditions increase levels heart would promote MCL‐1 cardiomyocyte Since Rapamycin (Rap) known to diabetes, we further posited Rap treatment (750 µg/kg/day; 6 weeks) suppress tissues Zucker Fatty (ZDF) rat, rodent model the progression Type 2 Diabetes. attenuated plasma insulin (INS) implying development insulinopenia; however, hyperglycemia persisted. q‐PCR showed significant miR‐29a, b, c (p<0.01) ZDF heart. also resulted loss cardiomyocytes as visualized histopathological analysis H & E Masson's trichrome staining. Moreover, increased suppressed mRNA mouse atrial (10nM; 12hr) human coronary artery smooth muscle cells (20nM; 12hr). These data suggest regulation via family INS‐responsive conserved across species contributes can failure diabetes. Grant Funding Source : NIH: 1R01HL118376‐01