Effect of chronic fish oil supplementation on renal function parameters of mice fed with a high fat or a high carbohydrate diet (689.9)

• DOI: 10.1096/fasebj.28.1_supplement.689.9
• Publication Date: 2021-06-15T17:18:06Z
• Authors: Ricardo Fernandez; Danielle Pequito; Isabela Coelho
• Keywords: Microalbuminuria

Fish oil (FO) supplementation, rich in n‐3 PUFA, has a positive effect on various diseases, including cancer, kidney diseases and diabetes. High fat (HFD) and/or high carbohydrate (HC) diets promote obesogenic effects induce insulin resistance.The objective of this study was evaluate the chronic fish supplementation renal function parameters mice fed with HFD or HC diets. Six weeks male were divided three groups different during eight weeks: regular rodent chow (S), HFD. After this, they supplemented orally for 30 days FO, 1g/kg body weight. Renal parameters, urinary excretion TXB2 PGE2, immunolocalization vimentin, desmin, α‐SMA PCNA, intraperitoneal glucose tolerance test measured all groups. Fasting levels significant higher vs S (139.6±6.2 mg/dL 104.5±5.5) (n=13).The group present intolerance, an that not modified by supplementation. Plasma similar between Glomerular filtration (creatinine clearance) lower (0.63±0.10ml/min/kg) (0.78±0.1) if compared to (1.58±0.4) (p<0.01, n=10). FO did induced changes parameter. Urinary also significantly (2.0±0.4pg/min) (2.3±0.6) than (11.3±3.3). The mean microalbuminuria level (expressed as urine albumin‐creatinine ratio) C 11.06±2.16ng/mg, value increased 18.24±2.74 35.65±7.32 partially reverted increase microalbumin (21.33±1.33).The intrarenal expression vimentin interstitium groups, tubules, In summary, alterations Grant Funding Source : Supported CAPES (Brazil)