Prepregnancy Vitamin D Deficiency and Placental Development in Mice

• DOI: 10.1096/fasebj.29.1_supplement.lb259
• Publication Date: 2021-06-21T16:57:50Z
• Authors: Alison Gernand; Margherita Cantorna; Francisco Diaz; Lindsay Snyder; James Hester; Sreelakshmi Vasud...; Manasi Kamat; Troy Ott
• Keywords: Litter

Maternal vitamin D deficiency is a public health problem worldwide and linked to numerous adverse pregnancy outcomes with placental origins. Our objective was examine the effect of prepregnancy on vascular development. We modeled using Cyp27B1 genetic knockout (KO) mouse no dietary (n=6); wild-type (WT) mice sufficient diet were control (n=6). All fed respective from 3 weeks age onward. At 8 age, mated genetically dissimilar males produce allogeneic embryos. Mice euthanized at 12.5 days gestation fetal morphometry measured. Placental mRNA estimated by qPCR for angiogenic proteins (angiopoeitin-1 (Ang-1), Ang-2, endothelial growth factor (VEGF), (PlGF)). KO had fewer viable fetuses compared WT (6.2 vs. 8.5, p=0.033). There statistically significant differences in or weights Ang-1, PIGF (all p>0.05). However, placentas exhibited 27% reduction VEGF expression (p=0.047, Figure 1). Fewer established after mating, yet study design did provide sample size adequately test this effect. Prepregnancy maternal caused lower litter among that became pregnant decreased abundance. Ongoing work examining mechanisms effects Support: Penn State College Health Human Development