Noninvasive Biomarker Candidates for Cadmium‐induced Nephrotoxicity by 2DE/MALDI‐TOF MS and SILAC/LC‐MS Proteomic Analyses

• DOI: 10.1096/fasebj.30.1_supplement.602.3
• Publication Date: 2023-11-26T16:52:35Z
• Authors: Aree Moon; Sun Young Kim; Hye Min Lee; Eun‐yi Ko; Kyeong Seok Kim; Hyung Sik Kim
• Keywords: Nephrotoxicity

Cadmium (Cd 2+ ) is a major environmental pollutant that induces cytotoxicity by heavy‐metal accumulation. Prolonged Cd exposure leads to cell damage oxidative stress mainly in the kidneys, critical organ for detoxification. In order identify reliable on invasive protein biomarkers ‐induced nephrotoxicity, we performed 2DE/MALDI‐TOF MS and SILAC/LC‐MS analyses using conditioned media (CM) of HK‐2 human kidney epithelial cells treated with CdCl 2 . Here, identified heat shock cognate 71 kDa isoform1 (HSPA8) α‐enolase (ENO1) as potential biomarker candidates evaluation nephrotoxicity. Treatment increased level HSPA8 CM lysates cells. The mRNA was also treatment, indicating transcriptional regulation. ENO1 CM, but not ‐treated did affect ENO1. We provide evidence increases were due death through stress. levels detected other nephrotoxic agents such HgCl , NaAsO cisplatin, amphotericin B, cyclosporine A. Urine tissues rats showed HSPA8. Taken together, this study ENO1as non‐invasive two comparative proteomic analyses. These new may have alternatives traditional efficient sensitive assessment Support or Funding Information This work supported grant from Korea Food & Drug Administration [10182KFDA992‐3202] National Research Foundation (NRF) grants [2013R1A2A2A04013379].