Anti‐inflammatory effect of caffeine by regulating NF‐κB activation in murine macrophage

• DOI: 10.1096/fasebj.30.1_supplement.lb256
• Publication Date: 2023-11-26T16:24:46Z
• Authors: Ji‐Hyun Hwang; Eun‐Jeong Koh; Yeon‐Joo Lee; Jia Chio; Ji‐hyeon Song; Young‐Jin Seo; Boo‐Yong Lee
• Keywords: Xanthine; Proinflammatory cytokine

Caffeine is white crystalline xanthine alkaloid and found in the seeds, coffee plant leaves of tea bush. In this study, we determined whether caffeine exerts anti‐inflammatory effects on lipopolysaccharide (LPS)‐induced inflammation RAW264.7 cells. To examine LPS‐induced inflammation, RAW 264.7 cells were treated with various concentrations presence or absence LPS. Our data showed that decreased inflammatory mediator, nitric oxide (NO). treatment also reduced expression pro‐inflammatory genes inducible synthase (iNOS), cyclooxygenase‐2 (COX‐2), interleukin (IL)‐3, IL‐6 IL‐12, secretion phosphorylated p38MAPK LPS‐treated inhibited nuclear translocation factor κB (NF‐κB) via IκBα phosphorylation. addition, NO production zebrafish. may inhibit responses murine macrophage by regulating NF‐κB activation MAPK