Behavioral Profile Assessment in Offspring of Swiss Mice Underwent to Neonatal Treatment with Fluoxetine

• DOI: 10.1096/fasebj.31.1_supplement.1076.11
• Publication Date: 2023-11-26T17:16:59Z
• Authors: Wellington da Sil...; Roberto Laureano‐...; Rodrigo Rodrigues...; Janaina Sena Souza; Raphael Silva Lau; Claudio da Silva ...; Gisele Giannocco
• Keywords: TPH2; Serotonin reuptake inhibitor; Tail suspension test

Serotonin exerts an important role in the central nervous system embryogenesis of mammals, influencing directly several brain systems ontogeny. Therefore, we investigated effect neonatal Fluoxetine (FLU), a selective serotonin reuptake inhibitor, on behavioral expression adult male Swiss mice. For this purpose, three groups (n=6 each, and ~35g) pregnant female mice were mated. The offspring obtained was treated with FLU (1mg/kg, s.c.) from 5 th at 15 post‐natal day. On 16 day, puppies euthanized hippocampus dissected for RNA analysis whereas offspring, by completing 70 days life, underwent assessment open field, light‐dark box tail suspension test. Statistical performed t‐Student means considered significantly different when p<0.05. According to our results, programmed animals had decrease TPH2 (0.07±0.007 vs. 1.07±0.19, p<0.001), 5HT1a (0.08±0.007 1.02±0.17, SERT (0.35±0.08 1.09±0.18, p=0.004), BDNF (0.19±0.02 p=0.001) LMX1B (0.03±0.01 1.12±0.25, expression. In addition, it observed less immobility time test (77.6±7.25 112.1±12.2, p=0.02) higher grooming field (7.6±1.4 3.1±0.9, p=0.01). test, showed shorter light side than control (36.69±10.4 76.2±5.48, p<0.01). These findings suggest that programming during period alters hippocampal serotonergic system, promoting anxiety depression‐like behaviors adults. Support or Funding Information FAPERJ & CNPq