Genomic analysis of smooth muscle cells in three‐dimensional collagen matrix

• DOI: 10.1096/fj.02-0256fje
• Publication Date: 2003-01-14T00:44:17Z
• Authors: Song Li; Jianmin Lao; Benjamin P.C. Chen; Yi‐shuan Li; Yihua Zhao; Julia Chu; Kuang‐Den Chen; Tsui‐Chun Tsou; Konan Peck; Shu Chien
• Keywords: Matrix (chemical analysis); PTK2

The proliferation, differentiation, and protein synthesis of vascular smooth muscle cells (SMCs) play important roles in remodeling. Here, we compared the genetic programming signaling SMCs collagen matrix as a three-dimensional (3-D) environment on two-dimensional (2-D) surface. By using DNA microarrays with 9600 genes, showed that 77 genes were expressed more than twofold 22 less one-half 3-D matrix, when 2-D condition. higher expression level cyclin-dependent kinase inhibitor 1 (p21) suggests p21 may be responsible for lower proliferation rate matrix. I was suggesting have increased synthesis. In addition, had stress fibers focal adhesions, tyrosine phosphorylation adhesion (FAK). Overexpression FAK attenuated functions molecular switch cell cycle regulation information generated this study helps to elucidate basis modulation SMC phenotypes by extracellular