Aging‐induced proinflammatory shift in cytokine expression profile in rat coronary arteries

• DOI: 10.1096/fj.02-1049fje
• Publication Date: 2003-04-29T00:13:44Z
• Authors: Anna Csiszar; Zoltan Ungvari; Akos Koller; John G. Edwards; Gabor Kaley
• Keywords: Proinflammatory cytokine; Coronary arteries

The phenotypic and functional changes of coronary arteries with aging promote ischemic heart disease. We hypothesized that these alterations reflect an aging-induced proinflammatory shift in vascular regulatory mechanisms. Thus, isolated young (3-month-old) aged (25-month-old) male Fischer 344 rats the expression 96 cytokines, chemokines, their receptors were screened by a cDNA-based microarray technique. In vessels expressions tumor necrosis factor (TNF)-alpha (3.3x), interleukin (IL)-1beta (3.0x), IL-6 (2.9x), IL-6Ralpha (2.8x) IL-17 (6.1x) genes significantly increased over vessels. Quantitative reverse transcriptase-polymerase chain reaction confirmed results. Western blotting demonstrated protein TNF-alpha, IL-1beta, IL-6, also compared those rats. Immunofluorescent double labeling showed IL-1beta are predominantly localized endothelium, whereas TNF-alpha smooth muscle. profile cytokine may contribute to arteries, promoting development disease elderly.