Mechanical stretch activates nuclear factor‐kappaB, activator protein‐1, and mitogen‐activated protein kinases in lung parenchyma: implications in asthma

• DOI: 10.1096/fj.02-1148com
• Publication Date: 2003-09-30T20:45:05Z
• Authors: Ashok Kumar; Savita Lnu; Rahul Malya; David Barron; Joan Moore; David B. Corry; Aladin M. Boriek
• Keywords: Parenchyma; Methacholine; Proinflammatory cytokine; c-jun

We investigated the effects of mechanical stretch and induced stimulation lung parenchyma on activation proinflammatory transcription factors in normal mice a mouse model asthma. Mechanical stretching led to increased NF-kappaB AP-1 factors. Incubation with methacholine NF-kappaB, which was further augmented by stretch. Activation response associated phosphorylation degradation IkappaBalpha IkappaB kinase. Stretch-induced involves stretch-activated (SA) channels production free radicals. and/or treatment resulted an ERK1/2 p38 MAP kinase, inhibition activity these kinases partially blocked stretch-induced activation. A greater level observed asthmatic mice, stretching. The cyclooxygenase-2, NF-kappaB-regulated enzyme, also higher from than mice. Our data suggest that activates AP-1, at least part, through kinase signaling pathways.