Anti‐inflammatory effects of hypoxia‐preconditioned human periodontal ligament cell secretome in an experimental model of multiple sclerosis: a key role of IL‐37

• DOI: 10.1096/fj.201700524r
• Publication Date: 2017-08-24T00:10:56Z
• Authors: Sabrina Giacoppo; Soundara Rajan Th...; Francesca Diomede; Placido Bramanti; Pio Conti; Oriana Trubiani; Emanuela Mazzon
• Keywords: Proinflammatory cytokine; Myelin oligodendrocyte glycoprotein

Recent research has widely investigated the anti-inflammatory effects of mesenchymal stem cells and their secretory products, termed secretome, in treatment multiple sclerosis (MS). The present study examined capacity conditioned medium (CM) from human periodontal ligament (hPLSCs) under hypoxia (H-hPDLSCs-CM) to suppress experimental autoimmune encephalomyelitis (EAE), a murine model MS. To induce EAE, female C57BL/6 mice were immunized with myelin oligodendroglial glycoprotein peptide35-55 At onset symptoms, H-hPDLSCs-CM was infused via tail vein mice. Our results demonstrate efficacy diminishing clinical histologic disease score. A key finding this is marked expression cytokine IL-37, paralleled by suppression proinflammatory cytokines EAE that treated H-hPDLSCs-CM. In addition, consequent modulation oxidative stress, autophagic, apoptotic markers observed after hPDLSCs-CM administration. provide additional evidence molecular mechanisms underlie H-hPDLSCs-CM, we its therapeutic action scratch injury-exposed NSC-34 neurons, an vitro injury. This reproduces severe inflammation stress conditions as damage. corroborate ability modulate inflammatory, pathways. Taken together, our findings suggest new pharmacologic opportunity for management MS.-Giacoppo, S., Thangavelu, S. R., Diomede, F., Bramanti, P., Conti, Trubiani, O., Mazzon, E. Anti-inflammatory hypoxia-preconditioned cell secretome sclerosis: role IL-37.