Enhanced osteogenic differentiation of human bone–derived mesenchymal stem cells in 3‐dimensional printed porous titanium scaffolds by static magnetic field through up‐regulating Smad4

• DOI: 10.1096/fj.201802195r
• Publication Date: 2019-02-14T19:00:45Z
• Authors: Yu He; Lingjia Yu; Jieying Liu; Yaqian Li; Yuanhao Wu; Zhenfei Huang; Di Wu; Hai Wang; Zhihong Wu; Guixing Qiu
• Keywords: Male; Titanium; 0301 basic medicine; Tissue Engineering; Tissue Scaffolds; Mesenchymal Stem Cells; Bone and Bones; Rats; Up-Regulation; Rats, Sprague-Dawley; 03 medical and health sciences; Magnetic Fields; Osseointegration; Osteogenesis; Printing, Three-Dimensional; Animals; Humans; Cells, Cultured; Smad4 Protein

The reconstruction of large bone defects remains a significant challenge for orthopedists. Three-dimensional-printed (3DP) scaffold is considered promising repair material. Static magnetic field (SMF) treatment an effective and noninvasive therapeutic method to improve regeneration. However, the osteogenic effect SMF on human bone-derived mesenchymal stem cells (hBMSCs) in 3DP scaffolds, as well its potential mechanism, are unclear. In this study, hBMSCs was investigated vitro vivo. addition, mechanism promoting osteogenesis by proteomic analysis. results showed that promoted differentiation vitro. A total 185 differential proteins were identified under conditions might be associated with morphogenetic protein-Smad1/5/8-signaling pathway increased transport phosphorylated Smad1/5/8 Smad2/3 nucleus up-regulating Smad4 conditions. vivo experiment regeneration osseointegration enhanced rat model defect. conclusion, moderate safe enhancing scaffolds vivo.-He, Y., Yu, L., Liu, J., Li, Wu, Huang, Z., D., Wang, H., Qiu, G. Enhanced 3-dimensional printed porous titanium static through Smad4.