Abstract 42: Human Adipose-Derived Stem Cells with BRCA Mutation Promote Breast Tumor Cell Migration

• DOI: 10.1097/01.gox.0000516563.61930.b8
• Publication Date: 2017-04-28T12:32:26Z
• Authors: Ruya Zhao; Irene J. Pien; Xinjian Liu; Victoria L. Seewaldt; Chuan-yuan Li; Scott T. Hollenbeck
• Keywords: 03 medical and health sciences; 0302 clinical medicine; PSRC 2017 Abstract Supplement

PURPOSE: We have previously shown that human breast adipose-derived stem cells (ASC) from BRCA mutation carriers behave differently non-mutation in regards to adipocyte differentiation and cytokine production. It is unknown if the impacts adipose tissue a manner promotes cancer. In this study we further investigate effects mutations on interaction between ASCs cancer cells. METHODS: BRCA1-knockout were generated using CRISPR/Cas9 system commercially available ASC lines. BRCA1 protein expression was assessed with Western blots. Next, conditioned medium groups without knockdown. media added MDA-MB-231/Luciferase cell lines (Luciferase-labeled triple negative tumor lines). Cancer proliferation evaluated luciferase assays. Scratch assays used monitor migration MDA-MB-231 incubated media. parallel studies, primary our repository also (n=8, 2 patients deleterious mutations, intron or clinical significance, 1 patient BRCA2 mutation, 3 genetic mutations). The assay scratch repeated samples. RESULTS: Using CRISPR/Cas9-mediated methods, able generate effective knockdown ASCs, as demonstrated by blot. Conditioned did not significantly alter comparison control (data shown). However, knockout showed higher rate ASCs. increased 24 hours vitro (p<0.00001). This effect observed for either mutations. CONCLUSIONS: demonstrate here first time potential increase migration. true both samples possessing mutation.