P51. Point-of-care Autologous Skin Cell Suspension for the Closure of Full-thickness Defects: Case Series of 40 Patients Treated at an Urban Level 1 Trauma Center

• DOI: 10.1097/01.gox.0001018472.77922.e5
• Publication Date: 2024-05-10T12:06:26Z
• Authors: Charles S. Hultman; Minakshi Pillai; Corianne Rogers; Carrie McGroarty; Samantha Brown; Michelle McMoon
• Keywords: Closure (psychology); Trauma Center; Suspension; Center (category theory)

PURPOSE: Autologous Skin Cell Suspension (ASCS) has documented success in closing partial-thickness burns, but less is known about the efficacy of ASCS full-thickness injuries. We examined utility defects. METHODS: Forty consecutive patients with defects (burns 7, MVC 12, infection 13, other 8) were treated bilayer technique 3:1 widely-meshed autograft and 80:1 expanded ASCS, at a Level 1 trauma center. Single-cell suspension keratinocytes, melanocytes, fibroblasts underwent point-of-care OR preparation using trypsinization, disaggregation, micro-filtration. Aerosolized cells sprayed over grafts donor sites, fixated by tissue glue. End points >99% re-epithelialization 4, 8, 12 weeks, limb salvage rate, site reduction, operative hospital throughput, incidence complications. RESULTS: Percentage near-total wound closure was 75%, 95%, 97.5% respectively. Limb occurred 35/36 (6 upper, 30 lower extremities). Mean area grafted 506 cm2, size 248 cm2 (51% reduction). surgical time 73 minutes; total 129 minutes. length-of-stay 23.1 days; from grafting to discharge 9.5 days. 3/40 (7.5%) required reoperation for bleeding (1) breakdown (2). 4/40 (10%) developed hypertrophic scarring. follow-up: 101.5 CONCLUSION: When used wounds, effective safe. Particular benefits include rapid re-epithelialization, high rate salvage, reduction morbidity, low