Vascular methylglyoxal metabolism and the development of hypertension

• DOI: 10.1097/01.hjh.0000173778.85233.1b
• Publication Date: 2005-07-07T11:24:53Z
• Authors: Xiaoxia Wang; Kaushik Desai; Tuanjie Chang; Lingyun Wu
• Keywords: Glycation End Products, Advanced; Male; Glutathione Peroxidase; 0303 health sciences; Age Factors; Hydrogen Peroxide; Pyruvaldehyde; Glutathione; Immunohistochemistry; Rats, Inbred WKY; Rats; Oxidative Stress; 03 medical and health sciences; Glutathione Reductase; Species Specificity; Superoxides; Rats, Inbred SHR; Hypertension; Animals; Aorta

The pathogenic process of diabetes mellitus is associated with increased methylglyoxal (MG). MG causes non-enzymic glycation proteins to form irreversible advanced endproducts (AGEs). However, the correlation between and essential hypertension unknown. aim present study was investigate whether MG, MG-induced AGEs, oxidative stress were in aorta spontaneously hypertensive rats (SHR) an formation related AGEs correlated development high blood pressure these rats.High-performance liquid chromatography (HPLC) used determine reduced glutathione levels plasma aorta. N(epsilon)-carboxyethyl-lysine (CEL) N(epsilon)-carboxymethyl-lysine (CML), determined using immunohistochemistry. Hydrogen peroxide superoxide peroxidase reductase activities also determined.Aortic significantly elevated SHR, but not Wistar-Kyoto (WKY) rats, at 8, 13 20 weeks age, parallel increase. Immunohistochemistry revealed more intense staining for CML CEL from SHR than those WKY 8 onwards. Most localized endothelial cells. Superoxide hydrogen weeks, whereas levels, decreased compared rats.Increased aortic AGE increase which may cause dysfunction altered vascular reactivity.