MP77-10 PROGRESSION AND FORMATION OF BACTERIAL BIOFILMS ON INDWELLING URINARY CATHETERS OVER TIME

• DOI: 10.1097/01.ju.0000557322.77175.6f
• Publication Date: 2019-04-24T05:40:57Z
• Authors: Glenn T. Werneburg*; Anh Nguyen; Nadine Henderson; Amanda Le Sueur; Anthony Corcoran; Aaron Katz; Jason Kim; Annie Rohan; David G. Thanassi
• Keywords: Urinary catheterization

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease the Genitourinary Tract: Kidney & Bladder II (MP77)1 Apr 2019MP77-10 PROGRESSION AND FORMATION OF BACTERIAL BIOFILMS ON INDWELLING URINARY CATHETERS OVER TIME Glenn T. Werneburg*, Anh Nguyen, Nadine Henderson, Amanda Le Sueur, Anthony Corcoran, Aaron Katz, Jason Kim, Annie Rohan, and David G. Thanassi Werneburg*Glenn Werneburg* More articles by this author , NguyenAnh Nguyen HendersonNadine Henderson SueurAmanda Sueur CorcoranAnthony Corcoran KatzAaron Katz KimJason Kim RohanAnnie Rohan ThanassiDavid View All Author Informationhttps://doi.org/10.1097/01.JU.0000557322.77175.6fAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION OBJECTIVES: Urinary catheter biofilm formation is common consists adherent bacteria, metabolic products, host components. Through biofilms, bacteria can resist both antibiotics defense mechanisms. Biofilm on urinary catheters a critical step in pathogenesis catheter-associated UTI. We sought determine locations onset progression formation. METHODS: 0-5 weeks indwelling time were collected. Catheters then analyzed using spectrophotometry. Specifically, sectioned stained with crystal violet, excess dye was removed. Adherent which represented biofilm, dissolved, concentration spectrophotometrically quantitated relative controls. RESULTS: 33 collected: 10 week 1, 6 2, 3 3, 8 4, 5. Biofilms progressed throughout all aspects over 5 time. The distal end exhibited significantly greater staining proximal side (Figure). balloon consistently showed intense staining, indicative significant adherence. CONCLUSIONS: as increased, detected following little 1 day greatest predominance portion well balloon. expect that these findings will inform design production novel catheters, may benefit from focusing antiseptic strategies portions catheter. Importantly, our suggest biofilms proliferate through multimodal etiology, sterile insertion technique prevent early formation, but not reduce growth. Ongoing studies bacterial species responsible for whether proliferation affected patient sex. Source Funding: GTW supported award T32GM008444 Award F30AI112252 NIH. Shaker Heights, OH; Stony Brook, NY; Mineola, NY© 2019 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e1136-e1136 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand PDF downloadLoading ...