Inhaled Nitric Oxide Attenuates the Adverse Effects of Transfusing Stored Syngeneic Erythrocytes in Mice with Endothelial Dysfunction after Hemorrhagic Shock

• DOI: 10.1097/aln.0b013e318272d866
• Publication Date: 2012-11-21T09:13:16Z
• Authors: Chong Lei; Binglan Yu; Mohd Shahid; Arkadi Beloiartsev; Kenneth D. Bloch; Warren M. Zapol
• Keywords: Endothelial Dysfunction

Background The authors investigated whether transfusion with stored erythrocytes would increase tissue injury, inflammation, oxidative stress, and mortality (adverse effects of transfusing erythrocytes) in a murine model hemorrhagic shock. They tested the adverse associated were exacerbated by endothelial dysfunction ameliorated inhaling nitric oxide. Methods studied mice fed high-fat diet (HFD-fed; to induce dysfunction) or standard for 4-6 weeks. Mice subjected 90 min shock, followed resuscitation leukoreduced syngeneic less than 24 h (fresh 2 weeks (stored erythrocytes). Results In standard-diet-fed at after resuscitation, increased injury more fresh erythrocytes. marked HFD-fed lactate levels short-term mortality. Compared erythrocytes, was reduction P50, plasma hemoglobin levels, indices inflammation that mice. Inhaled oxide reduced stress improved survival resuscitated Conclusions Resuscitation adversely impacts outcome an effect is dysfunction. reduces improves