Investigation of Dichotomizing Sensory Nerve Fibers Projecting to the Lumbar Multifidus Muscles and Intervertebral Disc or Facet Joint or Sacroiliac Joint in Rats

• DOI: 10.1097/brs.0b013e3182293178
• Publication Date: 2011-06-23T09:45:52Z
• Authors: Tomotaka Umimura; Masayuki Miyagi; Tetsuhiro Ishikawa; Hiroto Kamoda; Ken Wakai; Takafumi Sakuma; Ryo Sakai; Kazuki Kuniyoshi; Nobuyasu Ochiai; Shunji Kishida; Junichi Nakamura; Yawara Eguchi; Nahoko Iwakura; Tomonori Kenmoku; Gen Arai; Sumihisa Orita; Miyako Suzuki; Yoshihiro Sakuma; Gou Kubota; Yasuhiro Oikawa; Gen Inoue; Yasuchika Aoki; Tomoaki Toyone; Kazuhisa Takahashi; Seiji Ohtori
• Keywords: Male; Lumbar Vertebrae; Sensory Receptor Cells; Sacroiliac Joint; Axons; Zygapophyseal Joint; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; 0302 clinical medicine; Ganglia, Spinal; Animals; Intervertebral Disc; Muscle, Skeletal; Low Back Pain

Immunohistological analysis of dichotomizing sensory nerve fibers projecting to the lumbar multifidus muscles and intervertebral disc (IVD), facet joint (FJ), or sacroiliac joint (SIJ) in rats.To elucidate dichotomizing sensory nerve fibers projecting to the lumbar multifidus muscles and to IVDs, FJs, or SIJs.Clinically, the origin of low back pain remains unknown. Multiple studies have identified lumbar muscles, IVDs, FJs, and SIJs as sources of low back pain. Pain may originate directly from lumbar muscles or be referred from the spine, or both. Dorsal root ganglion (DRG) neurons with dichotomizing axons have been reported in several species and are thought to be related to referred pain.We used 2 neurotracers, 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) and fluorogold (FG), in this double-labeling study involving 30 Sprague Dawley rats. DiI was applied to lumbar multifidus muscles in all rats. Simultaneously, FG was applied to the anterior left portion of L5-L6 IVDs in the IVD group (n = 10), to the left L5-L6 FJs in the FJ group (n = 10), and to the left SIJs in the SIJ group (n = 10). Fourteen days after surgery, left DRGs from L1 to L6 were harvested, sectioned, and observed under a fluorescence microscope.We verified the existence of double-labeled DRG neurons (i.e., dichotomizing sensory nerve fibers) projecting to lumbar multifidus muscles and to IVDs, FJs, or SIJs, depending on the group. The proportion of double-labeled cells in all DiI-labeled DRG neurons was higher in the FJ group (6.8%) and SIJ group (7.1%) than in the IVD group (3.1%) (P < 0.05).Our results document the presence of dichotomizing sensory nerve fibers projecting to lumbar multifidus muscles and to IVDs, FJs, and SIJs. Referred low back muscle pain may reflect disorders of lumbar posterior structures, such as FJs and SIJs, rather than disorders of lumbar anterior structures, such as IVDs.