1′S-1′-acetoxyeugenol acetate

• DOI: 10.1097/cad.0b013e328343cbe6
• Publication Date: 2011-03-03T11:49:32Z
• Authors: Lionel Lian Aun; Mohamad Nurul Azmi; Halijah Ibrahim; Khalijah Awang; Noor Hasima Nagoor
• Keywords: 0301 basic medicine; Paclitaxel; QH301 Biology; Apoptosis; Breast Neoplasms; Cell Line; 03 medical and health sciences; Cell Line, Tumor; 616; Antineoplastic Combined Chemotherapy Protocols; Eugenol; Humans; Gene Expression Profiling; NF-kappa B; Drug Synergism; Epithelial Cells; Microarray Analysis; I-kappa B Kinase; 3. Good health; Gene Expression Regulation, Neoplastic; Alpinia; Female; Poly(ADP-ribose) Polymerases; Rhizome; Drugs, Chinese Herbal

In this study, the apoptotic mechanism and combinatorial chemotherapeutic effects of cytotoxic phenylpropanoid compound 1'S-1'-acetoxyeugenol acetate (AEA), extracted from rhizomes Malaysian ethnomedicinal plant Alpinia conchigera Griff. (Zingiberaceae), on MCF-7 human breast cancer cells were investigated for first time. Data assays such as live dead poly-(ADP-ribose) polymerase cleavage indicated that AEA was able to induce apoptosis in cells, but not normal mammary epithelial cells. A microarray global gene expression analysis treated with AEA, suggested induction tumor cell death through modulated dysregulation nuclear factor-kappaB (NF-κB) pathway, shown by reduced various κB-regulated targets. Consequent this, western blot proteins corresponding NF-κB pathway inhibited phosphorylation levels inhibitor κB-kinase complex, resulting elimination resistance originating activation. This AEA-based modulation elucidated time gave rise proposal an model termed 'Switching/Alternating Model.' addition also found synergistically enhance proapoptotic paclitaxel, when used combination presumably a chemosensitizing role. Therefore, it concluded isolated tropical ginger (A. conchigera) served very promising candidate further in-vivo development animal models subsequent clinical trials involving patients breast-related malignancies.