Cardiac and metabolic effects of hypothermia and inhaled hydrogen sulfide in anesthetized and ventilated mice*

• DOI: 10.1097/ccm.0b013e3181b9ed2e
• Publication Date: 2010-01-14T07:51:53Z
• Authors: Katja Baumgart; Florian Wagner; Michael Gröger; Sandra Weber; Eberhard Barth; Josef A. Vogt; Ulrich Wachter; Markus Huber-Lang; Markus W. Knöferl; Gerd Albuszies; Michael Georgieff; Pierre Asfar; Csaba Szabó; Enrico Calzia; Peter Radermacher; Vladislava Simkova
• Keywords: Male; 0301 basic medicine; [SDV]Life Sciences [q-bio]; Cell Respiration; Blood Pressure; Heart; Stroke Volume; Respiration, Artificial; 3. Good health; [SDV] Life Sciences [q-bio]; Mice, Inbred C57BL; Mice; Oxidative Stress; 03 medical and health sciences; Glucose; Heart Rate; Hypothermia, Induced; Administration, Inhalation; Animals; Anesthesia; Hydrogen Sulfide; Cardiac Output; Oxidation-Reduction

To test the hypothesis whether inhaled hydrogen sulfide amplifies effects of deliberate hypothermia during anesthesia and mechanical ventilation as is used to provide organ protection after brain trauma or circulatory arrest. Awake mice inhaling exhibit reduced energy expenditure, hypothermia, bradycardia despite unchanged systolic heart function. In rodents, alone causes decreased metabolic rate thus bradycardia.Prospective, controlled, randomized study.University animal research laboratory.Male C57/B6 mice.After surgical instrumentation (central venous, left ventricular pressure-conductance catheters, ultrasound flow probes on portal vein superior mesenteric artery), normo- hypothermic animals (core temperature = 38 degrees C 27 C) received either 100 ppm vehicle over 5 hrs (3 normothermia).During normothermia, had no hemodynamic effect. With without sulfide, blood pressure, rate, cardiac output, whereas stroke volume, ejection fraction, end-diastolic pressure remained unaffected. Myocardial hepatic oxidative deoxyribonucleic acid damage (comet assay) endogenous glucose production (rate appearance 1,2,3,4,5,6-13C6-glucose) were similar in all groups. Hypothermia comparably CO2 with sulfide. During increased oxidation (derived from expiratory 13CO2/12CO2 ratio). This shift toward preferential carbohydrate utilization coincided a significantly attenuated responsiveness mitochondrial respiration stimulation exogenous cytochrome-c-oxidase (high-resolution respirometry).In anesthetized mechanically ventilated mice, did not amplify systemic alone. The aerobic together cellular cytochrome-c suggest that, improved yield respiration, possibly via maintenance integrity. Hence, may offer benefit therapeutic hypothermia.