The Marginating-pulmonary Immune Compartment in Rats: Characteristics of Continuous Inflammation and Activated NK Cells

• DOI: 10.1097/cji.0b013e3181b0b146
• Publication Date: 2009-12-17T15:24:55Z
• Authors: Rivka Melamed; Ella Rosenne; Marganit Benish; Yael Goldfarb; Ben Levi; Shamgar Ben-Eliyahu
• Keywords: Cytotoxicity, Immunologic; Inflammation; Male; 0303 health sciences; Interferon Inducers; Cell Separation; Neoplasms, Experimental; Flow Cytometry; Rats, Inbred F344; Rats; 3. Good health; Killer Cells, Natural; 03 medical and health sciences; Phenotype; Poly I-C; Animals; Lung

A significant role has been indicated for cellular immunity in controlling circulating cancer cells, but most autologous tumor cells seem resistant, vitro, to natural killer cell (NKC) and cytotoxic T lymphocytes cytotoxicity. Addressing this apparent contradiction, we recently identified a unique leukocyte population, marginating-pulmonary (MP)-leukocytes, which exhibit potent (NK) Here, characterize the MP-compartment naive immunostimulated rats, assessed its cytotoxicity against “NK-resistant” tumors cells. Animals were treated with poly I-C (3×0.2 mg/kg) or saline, circulating-leukocytes MP-leukocytes collected analyzed terms of composition, activation markers, NK leukocytes purified NKCs. Compared circulating-leukocytes, showed greater proportion granulocytes, monocytes, NKCs, large NKCs; higher expression adhesion markers (CD25, CD11a, CD11b, NKR-P1, IFN-γ); elevated NKCs several syngeneic xenogeneic NK-resistant target (from both F344 BDX inbred rats). In animals (treated I-C), not animals, from markedly superior cytotoxicity, suggesting that immunostimulation uniquely affect MP-NKCs, other support Overall, results suggest is characterized by continuous activated inflammatory microenvironment affected immunostimulation. If similarly MP-NKCs exist patients, then are considered could actually be controlled MP-NKCs. Innate may assume malignant spread, especially after