Induction of Specific Cellular and Humoral Responses Against Renal Cell Carcinoma After Combination Therapy With Cryoablation and Granulocyte-Macrophage Colony Stimulating Factor

• DOI: 10.1097/cji.0b013e31821dcba5
• Publication Date: 2011-05-14T11:08:19Z
• Authors: Archana Thakur; Peter Littrup; Elyse N. Paul; Barbara Adam; Lance K. Heilbrun; Lawrence G. Lum
• Keywords: Cellular immunity

Cryotherapy offers a minimally invasive treatment option for the management of both irresectable and localized prostate, liver, pulmonary, renal tumors. The antineoplastic effects cryotherapy are mediated by direct tumor lysis indirect effects, such as intracellular dehydration, pH changes, microvascular damage resulting in ischemic necrosis. In this study, we investigated whether percutaneous cryoablation lung metastasis from cell carcinoma (RCC) combination with aerosolized granulocyte-macrophage colony stimulating factor can induce systemic cellular humoral immune responses 6 patients RCC. Peripheral blood mononuclear cells (PBMCs) were sequentially studied up to 63 days post cryoimmunotherapy (CI). PBMC pre CI phenotyped lymphocyte subsets tested cytotoxicity interferon-γ EliSpots directed at RCC cells. Humoral measured vitro antibody synthesis assay monitoring data showed that induced specific cytotoxic T lymphocyte, antitumor responses, enhanced Th1 cytokine production 4 patients. More importantly, magnitude response seems be associated clinical responses. These pilot show robust brisk metastatic RCC, but requires further evaluation optimized protocols.