MicroRNA-199a-3p Regulates Endometrial Cancer Cell Proliferation by Targeting Mammalian Target of Rapamycin (mTOR)

• DOI: 10.1097/igc.0b013e31829ea779
• Publication Date: 2013-07-11T12:26:26Z
• Authors: Dong Wu; Hui-juan Huang; Chun-ni He; Kai-yu Wang
• Keywords: RPTOR; Sirolimus

Abnormal expression of miR-199a-3p, which has similar effects to oncogenes or tumor suppressor genes, can occur in various malignant tumors and is closely linked with cell proliferation, invasion, metastasis. However, its endometrial endometrioid adenocarcinoma (EEC) are still unclear. This study was designed identify the impact miR-199a-3p on proliferation EEC cells role carcinogenesis EEC.The levels paired adjacent nontumor tissues were analyzed by real-time polymerase chain reaction. The cycle apoptosis Ishikawa transfected mimics inhibitors. target genes predicted using bioinformatics methods. extent regulation determined luciferase reporter assays, Western blotting, quantitative pretreated gene-specific inhibitors further relationship between genes.Compared normal endometrium, reduced found human specimens. Compared control group microRNA mimics, proliferative capacity inhibited, whereas showed increased proliferation. inhibitory effect associated populations at G1-phase, decreased S-phase. results demonstrated that could inhibit protein mammalian rapamycin (mTOR) targeted binding mTOR-3' untranslated region. Inhibition mediated mTOR.MiR-199a-3p inhibits through negative mTOR expression. Restoration intracellular may serve as a potential option for treatment.