Cytoskeletal Changes of Mesenchymal Stem Cells During Differentiation

• DOI: 10.1097/mat.0b013e31802deb2d
• Publication Date: 2007-03-15T07:51:49Z
• Authors: Gregory Yourek; Mohammad A. Hussain; Jeremy J. Mao
• Keywords: Cytochalasin D; Cytochalasin B

Mesenchymal stem cells (MSCs) are progenitors for tissues such as bone and cartilage. In this report, the actin cytoskeleton nanomechanobiology of human mesenchymal (hMSCs) were studied using fluorescence microscopy atomic force (AFM). Human MSCs differentiated into chondrocytes osteoblasts per previous approaches. Cytochalasin D (CytD) was used to temporarily disrupt in hMSCs, hMSC-chondrocytes (hMSC-Cys) hMSC-osteoblasts (hMSC-Obs). Fluorescence revealed a dose-dependent response CytD. Removal CytD from media cytoskeleton-disrupted led recovery cytoskeletal structures, confirmed by both AFM. Force-volume imaging AFM evaluated nanomechanics all three cell types before, during, after treatment. disruption had marked effects on hMSCs hMSC-Cys, comparison with limited hMSC-Obs, qualitatively quantitatively Treatment resulted morphology changes types, significant decreases observed Young's Moduli hMSC-Cys. These data suggest alter their components during differentiation. Additional studies will address mechanisms biochemical biophysical methods.