Characterization of static adhesion of human platelets in plasma to protein surfaces in microplates

• DOI: 10.1097/mbc.0b013e328327353d
• Publication Date: 2009-05-21T10:31:12Z
• Authors: Andreas C Eriksson; Per A Whiss
• Keywords: Adenosine diphosphate; Platelet adhesiveness

Platelet adhesion is a complex and important event for prevention of blood loss after vessel injury. This study investigated fundamental adhesive mechanisms occurring in an in-vitro assay developed the measurement static human platelets plasma. The aim was to gain methodological knowledge that could be used interpretations results from other studies using this specific assay. Involvement receptors by use various antibodies as well therapeutic drugs (abciximab, eptifibatide tirofiban). Inhibitors adenosine 5′-diphosphate (cangrelor, MRS2179) thromboxane A2 signalling (BM-531) were estimate role autocrine activation. Adhesion collagen found mainly mediated α2β1 some extent αIIbβ3. fibrinogen In addition, 5′-diphosphate-induced albumin dependent on Furthermore, experiments with cangrelor BM-531 showed majority interactions tested or A2. We conclude measured platelet are accordance current regarding activation adhesion. Despite its simplicity, we suggest screening device influence surfaces soluble substances