Macrophage migration inhibitory factor expression and MIF gene −173 G/C polymorphism in nonalcoholic fatty liver disease

• DOI: 10.1097/meg.0b013e328331a596
• Publication Date: 2010-01-08T08:46:01Z
• Authors: Murat Akyildiz; Fulya Gunsar; Deniz Nart; Osman Sahin; Funda Yilmaz; Sinan Akay; Galip Ersoz; Zeki Karasu; Tankut Ilter; Yucel Batur; Afig Berdeli; Ulus Akarca
• Keywords: Adult; Male; Genotype; Biopsy; Polymerase Chain Reaction; Severity of Illness Index; 03 medical and health sciences; Gene Frequency; Nonalcoholic fatty liver disease; Humans; Macrophage Migration-Inhibitory Factors; Macrophage migration inhibitory factor; 0303 health sciences; Polymorphism, Genetic; Gene polymorphism; Middle Aged; Immunohistochemistry; Up-Regulation; 3. Good health; Fatty Liver; Intramolecular Oxidoreductases; Phenotype; Liver; Case-Control Studies; Female

To investigate the macrophage migration inhibitory factor (MIF) expression and -173 G/C polymorphism of the MIF gene in nonalcoholic fatty liver disease (NAFLD).Ninety-one patients with diagnosis of NAFLD and 104 healthy controls were included in the study. MIF -173 G/C polymorphism was detected using the PCR-restriction fragment length polymorphism based method. NAFLD was stratified as nonalcoholic steatohepatitis (NASH), probable NASH and steatosis, respectively in groups 1, 2 and 3, according to NAFLD Activity Score. MIF expression was detected by immunohistochemistry staining.Mean age of the patients was 50.1+/-9.6 years, and 54 of them were male. Serum alanine aminotransferase and aspartate aminotransferase were 50/83, 42/63 and 31/32, respectively in groups 1, 2 and 3, (P<0.05). Both the MIF expression of hepatocytes and mononuclear cells were more prominent in groups 1 and 2 than group 3. There was no correlation between MIF expression of hepatocytes and fibrosis stage. However, MIF expression of mononuclear cells significantly increased according to fibrosis stage (P<0.05, R : 0.2). There was no significant correlation between MIF genotype and MIF expression in the liver.MIF expression is significantly increased especially by mononuclear cells in liver tissue of patients with NASH secondary to inflammation. Thus, it should be considered as a consequence not a causal factor.