Aberrant Expressions of AP-2α Splice Variants in Pancreatic Cancer

• DOI: 10.1097/mpa.0b013e31821f2715
• Publication Date: 2011-06-08T09:50:42Z
• Authors: Catherine Carrière; Sarah Mirocha; Sophie Deharvengt; Jason R. Gunn; Murray Korc
• Keywords: HeLa; Transcription

Objectives: The present study was conducted to evaluate the expression and function of AP-2α isoforms in pancreatic ductal adenocarcinoma. Methods: evaluated at RNA level by reverse transcription-polymerase chain reaction protein Western blotting immunofluorescence. Its as a transcription factor transient transfection experiments: DNA binding properties electromobility shift assay transactivation capabilities luciferase assay. Results: Multiple alternative splicing events messenger occurred all human cancer cell lines, including novel isoform, termed variant 6, which not HeLa cells. At level, except for 1 line, lines expressed high nuclear levels AP-2α. We also showed that could bind its cognate recognition site activate transcription. However, although able transcription, did act dominant negative manner when cotransfected with full-length protein. Conclusions: are highly new seems be specific is deprived transcriptional activity.