Utility of GATA3 Immunohistochemistry in Differentiating Urothelial Carcinoma From Prostate Adenocarcinoma and Squamous Cell Carcinomas of the Uterine Cervix, Anus, and Lung

• DOI: 10.1097/pas.0b013e318260cde7
• Publication Date: 2012-09-15T04:06:08Z
• Authors: Alex Chang; Ali Amin; Edward Gabrielson; Peter Illei; Richard B. Roden; Rajni Sharma; Jonathan I. Epstein
• Keywords: Adenosquamous carcinoma; Tissue microarray; GATA3

Distinguishing invasive high-grade urothelial carcinoma (UC) from other carcinomas occurring in the genitourinary tract may be difficult. The differential diagnosis includes prostatic adenocarcinoma, spread an anal squamous cell (SCC), or a uterine cervical SCC. In terms of metastatic UC, most common problem is differentiating UC to lung primary pulmonary Immunohistochemical analysis (IHC) for GATA binding protein 3 (GATA3), thrombomodulin (THROMBO), and uroplakin III was performed on tissue microarray (TMA) containing 35 cases UC. GATA3 IHC also TMAs 38 (Gleason score ≥8) adenocarcinomas, representative sections 15 SCCs, 19 with 12 cervix [SCC (n=10), SCC neuroendocrine features (n=1), adenosquamous (n=1)]. addition, metastases TMA 25 SCCs 5 non-small features. GATA3, THROMBO, were positive 28 (80%), 22 (63%), 21 (60%) respectively. All GATA3-positive staining nonfocal; (89%) demonstrated moderate strong staining, (11%) weak staining. Of 7 that failed express THROMBO and/or III, whereas 2 negative all markers. None adenocarcinomas GATA3. Weak present occasional basal cells benign prostate glands, few atrophic metaplasia. (7%) showed focal 1 (3%) rarely observed epithelium. 31 carcinomas, 6 (19%) (3 nonfocal focal), (6%) Twelve (80%) UCs 11 showing diffuse case positive. sensitive marker typically intensity. highly specific excluding adenocarcinoma. Although some can positive, unlike more commonly weak. useful maker when diagnosing lung.