The Mechanism of Action of the Vacuum-Assisted Closure Device

• DOI: 10.1097/prs.0b013e31818237ac
• Publication Date: 2008-08-13T07:08:22Z
• Authors: Sandra Saja Scherer; Giorgio Pietramag...; Jasmine C. Mathews; Michael J. Prsa; Sui Huang; Dennis P. Orgill
• Keywords: Vascularity; Negative-pressure wound therapy; Closure (psychology); Vacuum aspiration; CD31

The vacuum-assisted closure device is widely used clinically, yet its mechanisms of action are incompletely understood. In this study, the authors designed a partially splinted full-thickness murine model to better understand mechanism device.Full-thickness wounds (n = 10 per group) were excised in diabetic mice and treated with or isolated components: an occlusive dressing, subatmospheric pressure at 125 mmHg (suction), polyurethane foam without downward compression. Results quantified two-dimensional immunohistochemical staging system based on blood vessel density (CD31) cell proliferation (Ki67) 7 days after wounding. Microscopic strain was measured by fixing situ all dressing modalities.Wounds exposed compressed uncompressed dressings showed 2-fold increase vascularity compared group (p < 0.05). addition stimulated proliferation, up 82 percent Ki67-positive nuclei, other groups. Direct measurements wound surface deformations significant microstrains groups (60 16 percent, respectively) groups.These data provide profound insights into device, providing explanation for increases bed components. suggest that vascular response related foam, whereas tissue strains induced proliferation.