Bone Marrow–Derived Mesenchymal Stem Cells Enhanced Diabetic Wound Healing through Recruitment of Tissue Regeneration in a Rat Model of Streptozotocin-Induced Diabetes

• DOI: 10.1097/prs.0b013e3182174329
• Publication Date: 2011-10-04T18:40:04Z
• Authors: Yur-Ren Kuo; Chun-Ting Wang; Jiin-Tsuey Cheng; Feng-Sheng Wang; Yuan-Cheng Chiang; Ching-Jen Wang
• Keywords: Stem Cell Therapy

Background: This study investigated whether bone marrow–derived mesenchymal stem cell therapy has effectiveness in the enhancement of diabetic wound healing through tissue regeneration. Methods: The authors used a dorsal skin defect (6 × 5 cm) streptozotocin-induced diabetes rodent model. Forty male Wistar rats were divided into four groups: group I, nondiabetic (controls); II, controls receiving no cells; III, 1 107 cells per dose (subcutaneously administered eight areas surrounding margin) on day 7; and IV, days 7 10. Wound was assessed clinically. Histologic examination performed with hematoxylin eosin staining. CD45, Ki-67, prolyl 4-hydroxylase, epidermal growth factor, vascular endothelial factor evaluated immunohistochemical analysis. Results: Overall clinical results showed that size significantly reduced cell–treated as compared controls. Complete wound-healing time statistically shorter treated once (6.6 ± 1.13 weeks versus 9.8 0.75 weeks; p < 0.001). It twice (5.2 6.6 = 0.026). analysis revealed significant reduction topical proinflammatory reaction suppression CD45 expression control group. On immunohistochemistry analysis, increases Ki-67 noted Conclusions: Mesenchymal enhanced healing. Treatment them is associated biomarkers