Decellularized Tracheal Matrix Scaffold for Tissue Engineering

• DOI: 10.1097/prs.0b013e31825dc084
• Publication Date: 2013-01-02T17:39:01Z
• Authors: Mengqing Zang; Qixu Zhang; Edward I. Chang; Anshu B. Mathur; Peirong Yu
• Keywords: Decellularization; Matrix (chemical analysis); Antigenicity

Background: A tracheal matrix scaffold decellularized by detergent-enzymatic treatment has been shown as a promising in tissue engineering. The objectives of this study were to evaluate the impact technique on extracellular integrity and characterize environment for recellularization. Methods: Brown Norway rat tracheae using modified treatment. Antigenicity cellularity monitored during processing. Glycosaminoglycan content, histoarchitecture, mechanical properties also evaluated. Matrix compatibility was determined cytotoxicity assay. Surface ultrastructure its interaction with seeded bone marrow stem cell–derived chondrocytes epithelial cells examined scanning electron microscopy. Results: Rat trachea treated five cycles demonstrated complete elimination antigenicity. Although there significant loss glycosaminoglycan (t test, p < 0.01), histoarchitecture cartilage basement membrane retained after decellularization. Stiffness decreased, but sufficient compressive strength preserved maintain lumen patency. showed good cell favored adhesion growth respiratory cells, Conclusions: At point antigen removal, altered composition major structure adequate strength. provided compatible supportive