Bone Regeneration Using a Bone Morphogenetic Protein-2 Saturated Slow-Release Gelatin Hydrogel Sheet

• DOI: 10.1097/sap.0b013e31819b6c52
• Publication Date: 2010-03-18T09:37:42Z
• Authors: Shinichi Asamura; Yuichi Mochizuki; Masaya Yamamoto; Yasuhiko Tabata; Noritaka Isogai
• Keywords: Gelatin

Bone regeneration methods using bone inductive cytokines show promise, however, due to early diffusion and absorption of single applications these cytokines, the effects are limited. In this study, such a system was applied, gelatin hydrogel as carrier slowly release (bone morphogenetic proteins) BMP-2 over relatively long period in vivo. By coupling slow-release with biodegradable copolymer, composite evaluated by grafting into defect sites canine orbital floor fracture model. Radio-iodinated incorporated subcutaneously implanted nude mice showed similar slow (approximately, 60% at 3 days 80% 14 days) radiolabeled alone. contrast, greater than 90% fluid-injected lost injection site within first 8 hours. Using dog model fracture, complex BMP-2-saturated polylactide-based copolymer defect. structural analysis, radiography, histologic examination, microfocus CT, greatly enhanced new formation healing 5 weeks comparison directly saturated same amount (no carrier). A trabecular structure resembling that normal tissue restored generated constructs. Thus study demonstrates potential for difficult defects.